Ikhithi yokukhipha i-Nucleic Acid (A02)
Umgomo wokutholwa
Ngemva kokukhipha i-DNA ye-genomic ngokuhlukanisa amaseli nge-lysis buffer, ubuhlalu obuzibuthe bungabopha ngokukhetha i-genomic DNA kusampula.Inani elincane lokungcola elimuncwa ubuhlalu obuzibuthe lingasuswa isigcinalwazi sokugeza.Ku-TE, ubuhlalu obuzibuthe bungakhulula i-DNA ye-boundgenome, buthole i-DNA yezinga eliphezulu yofuzo.Le ndlela ilula futhi iyashesha futhi izinga le-DNA ekhishiwe liphezulu, elingahlangabezana nemfuneko yokutholwa kwe-DNA methylation.Ngaleso sikhathi, ikhithi yokukhipha esekelwe kubuhlalu obuzibuthe ingase ihambisane nokukhipha i-nucleic acid okuzenzakalelayo, ukuhlangabezana nemisebenzi yokukhipha i-nucleic acid ephezulu.
Izingxenye eziyinhloko ze-reagent
Izingxenye ziboniswa kuthebula 1:
Ithebula 1 Izingxenye Ze-reagent futhi Iyalayisha
| Igama lengxenye | Izingxenye eziyinhloko | Usayizi (48) | Usayizi (200) |
| 1. Isivikelo Sokugaya A | Tris, SDS | 15.8 mL/ibhodlela | 66mL/ibhodlela |
| 2. ULysis Buffer L | I-Guanidinium Isothiocyanate, i-Tris | 15.8 mL/ibhodlela | 66mL/ibhodlela |
| 3. Geza Isivikelo A | NaCl, Tris | 11 mL/ibhodlela | 44 mL/ibhodlela |
| 4. Geza Isivikelo B | NaCl, Tris | 13 mL/ibhodlela | 26.5mL/ibhodlela *2 |
| 5. TE | Tris, EDTA | 12 mL/ibhodlela | 44 mL/ibhodlela |
| 6. Isixazululo se-Protease K | UProtease K | 1.1mL/ucezu | 4.4mL/ucezu |
| 7. Ukumiswa kobuhlalu obuzibuthe 2 | Ubuhlalu kazibuthe | 0.5mL/ucezu | 2.2mL/ucezu |
| 8. Imiyalo yokukhipha ama-nucleic acid reagent | / | 1 ikhophi | 1 ikhophi |
Izingxenye ezidingekayo ekukhishweni kwe-nucleic acid, kodwa azifakiwe kukhithi:
1. I-Reagent: I-ethanol e-anhydrous, isopropanol, ne-PBS;
2. Izinto ezisetshenziswayo: 50mL centrifuge tube kanye ne-1.5mL EP tube;
3. Imishini: Ukugeza kwamanzi, ama-pipettes, ishalofu kazibuthe, i-centrifuge, ipuleti lomthombo we-96 (okuzenzakalelayo), imishini yokukhipha i-nucleic acid ezenzakalelayo (okuzenzakalelayo).
Ulwazi oluyisisekelo
Izimfuneko zesampula:
1.Ukutholwa kuzoqedwa ngaphansi kwesitoreji sezinsuku ezingu-7 sezinga lokushisa le-ambient ngemva kokuqoqwa kwesampula yeseli ekhishwe ngomlomo wesibeletho (engagxilile).
2.Ukutholwa kuzoqedwa ngaphansi kwesitoreji sezinsuku ezingu-30 sezinga lokushisa le-ambient ngemva kokuqoqwa kwesampula yeseli ekhishwe ngomlomo wesibeletho (elungisiwe).
3.Ukutholwa kuzoqedwa ngaphansi kokugcinwa kwezinsuku ezingama-30 kwezinga lokushisa elizungezile ngemva kokuqoqwa kwesifanekiso somchamo;Ukutholwa kuzoqedwa ngesikhathi ngemuva kokuqoqwa kwamasampula eseli akhulisiwe.
Ukucaciswa kokupaka:200pcs/ibhokisi, 48pcs/ibhokisi.
Izimo zesitoreji:2-30 ℃
Isikhathi sokuqinisekisa:Izinyanga ezingu-12
Idivayisi esebenzayo:I-Tianlong NP968-C ithuluzi lokukhipha i-nucleic acid, ithuluzi lokukhipha i-nucleic acid ye-Tiangen TGuide S96, i-GENE DIAN EB-1000 ithuluzi lokukhipha i-nucleic acid.
Isitifiketi serekhodi ledivayisi yezokwelapha Inombolo/imfuneko yobuchwepheshe yomkhiqizo No.:HJXB No. 20210100.
Usuku lokugunyazwa nokubuyekezwa kwemiyalelo:Idethi yokugunyaza: Nov. 18, 2021
Mayelana NATHI
Njengebhizinisi lobuchwepheshe obuphezulu elasungulwa ngo-2018 ngochwepheshe abaphezulu be-epigenetic, i-Epiprobe igxile ekuxilongweni kwamangqamuzana omdlavuza we-DNA methylation kanye nemboni ye-theranostics enembile.Ngesisekelo sobuchwepheshe esijulile, sihlose ukuhola inkathi yemikhiqizo emisha ukuze inqamule umdlavuza!
Ngokusekelwe ocwaningweni lwesikhathi eside lweqembu eliyinhloko le-Epiprobe, ukuthuthukiswa kanye noguquko emkhakheni we-DNA methylation ngezinto ezintsha ezisezingeni eliphezulu, ezihlanganiswe nezinjongo eziyingqayizivele ze-DNA methylation zomdlavuza, sisebenzisa i-algorithm eyingqayizivele ehlanganisa idatha enkulu kanye nobuchwepheshe bobuhlakani bokwenziwa ukuze thuthukisa ngokuzimela ubuchwepheshe be-biopsy obuwuketshezi obuvikelwe ngelungelo lobunikazi.Ngokuhlaziya izinga le-methylation lezindawo ezithile zezingcezu ze-DNA zamahhala kusampula, ukushiyeka kwezindlela zokuhlola zendabuko kanye nemikhawulo yokuhlinzwa kanye nesampula yokubhoboza kuyagwenywa, okungaphumeleli nje kuphela ukutholwa okunembile komdlavuza wakuqala, kodwa futhi kwenza ukuqapha kwesikhathi sangempela. kokuvela komdlavuza kanye namandla okuthuthuka.
