I-TAGMe DNA Methylation Detection Kits (qPCR) ye-Endometrial Cancer
IZICI ZOMKHIQIZO
Ukunemba
Kugunyazwe amasampula omtholampilo angaphezu kuka-800 ocwaningweni lwezikhungo eziningi ezingaboni kabili, umkhiqizo unokucaciswa okungu-82.81% kanye nokuzwela okungama-80.65%.
Elula
Ubuchwepheshe bokuqala bokutholwa kwe-Me-qPCR bungaqedwa ngesinyathelo esisodwa phakathi namahora angu-3 ngaphandle kokuguqulwa kwe-bisulfite.
Ngaphambili
Iyabonakala esigabeni esiyingozi.
Okuzenzakalelayo
Isebenza ngebhulashi lomlomo wesibeletho kanye namasampula e-Pap smear.
UKUSETSHENZISWA OKUHLOSIWE
Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative ye-hypermethylation yofuzo lwe-PCDHGB7 izifanekiso zomlomo wesibeletho.Umphumela omuhle ubonisa ukwanda kwengozi yezilonda ze-endometrial precancerous kanye nomdlavuza, okudinga ukuhlolwa okuqhubekayo kwe-histopathological ye-endometrium.Ngokuphambene nalokho, imiphumela yokuhlolwa engemihle ibonisa ukuthi ingozi yezilonda ze-endometrial precancerous kanye nomdlavuza iphansi, kodwa ingozi ayikwazi ukukhishwa ngokuphelele.Ukuxilongwa kokugcina kufanele kusekelwe emiphumeleni yokuhlolwa kwe-histopathological ye-endometrium.I-PCDHGB7 iyilungu lomndeni we-protocadherin γ gene cluster.I-Protocadherin itholwe ukuthi ilawula izinqubo zebhayoloji ezifana nokwanda kwamangqamuzana, umjikelezo wamaseli, i-apoptosis, ukuhlasela, ukufuduka kanye ne-autophagy yamangqamuzana e-tumor ngokusebenzisa izindlela ezihlukahlukene zokubonisa, futhi ukuthuliswa kofuzo okubangelwa i-hypermethylation yesifunda somgqugquzeli kuhlobene eduze nokwenzeka nokuthuthuka. eminingi yomdlavuza.Kubikwa ukuthi i-hypermethylation ye-PCDHGB7 ihlotshaniswa nezinhlobonhlobo zezimila, njenge-non-Hodgkin lymphoma, umdlavuza webele, umdlavuza womlomo wesibeletho, umdlavuza we-endometrial kanye nomdlavuza wesinye.
ISIMISO SOKUTHOLA
Le khithi iqukethe i-nucleic acid extract reagent kanye ne-PCR yokuthola i-reagent.I-Nucleic acid ikhishwa ngendlela esekelwe ku-magnetic-bead.Le khithi isekelwe kumgomo wendlela ye-PCR yobuningi be-fluorescence, kusetshenziswa ukusabela kwe-PCR ye-methylation-specific real-time ukuhlaziya i-DNA yesifanekiso, kanye nokuthola amasayithi e-CpG yofuzo lwe-PCDHGB7 kanye nomaka wokulawula ikhwalithi wangaphakathi wezingcezu zofuzo lwereferensi i-G1 ne-G2.Izinga le-methylation le-PCDHGB7 kusampula, noma inani le-Me, libalwa ngokuvumelana nevelu ye-Ct ye-PCDHGB7 yofuzo lwe-methylated DNA amplification kanye nenani le-Ct lereferensi.I-PCDHGB7 gene hypermethylation isimo esihle noma esingalungile sinqunywa ngokwevelu Yami.
Izimo zohlelo lokusebenza
Ukuhlolwa kusenesikhathi
Abantu abanempilo
Ukuhlolwa Kwengozi Yomdlavuza
Amaqembu asengozini enkulu (abantu abanokopha okungavamile kwesitho sangasese sowesifazane ngemva kokuya esikhathini, ukujiya kwe-endometrial, njll.)
Ukuqapha Ukuphindaphinda
Inani labantu ababikezelayo
Ukubaluleka komtholampilo
Ukuhlolwa kusenesikhathi kwabantu abanempilo:Umdlavuza we-Endometrial kanye nezilonda ze-precancerous zingahlolwa ngokunembile;
Ukuhlolwa kobungozi kubantu abasengozini enkulu:Ukuhlolwa kwengozi kungenziwa kubantu abanokopha okungavamile kwesitho sangasese sowesifazane kanye nokuqina kwe-endometrial ngemva kokunqamuka kokuya esikhathini ukuze basize ekuxilongeni emtholampilo;
Ukuqapha okuphindaphindayo kwesibalo sabantu:Ukuqapha kokuphindaphinda kwenani labantu kwangemva kokuhlinzwa kungenziwa ukuze kuvinjelwe ukubambezeleka kokwelashwa okubangelwa ukuphindaphinda.
Iqoqo lesampula
Indlela yesampula: Beka isampula yomlomo wesibeletho esilahlwayo endaweni yomlomo wesibeletho, hlikihla kahle ibhulashi lomlomo wesibeletho bese uphendukisa izikhathi ezi-4-5 ngokwewashi, khipha kancane ibhulashi lomlomo wesibeletho, ulifake kusisombululo sokulondoloza amaseli, bese ulilebula ukuze kuhlolwe okulandelayo.
Ukulondolozwa kwamasampuli:Amasampuli angagcinwa ekamelweni lokushisa kuze kube yizinsuku eziyi-14, ku-2-8 ℃ kuze kufike ezinyangeni ezi-2, futhi ku-20±5℃ kuze kufike ezinyangeni ezingu-24.
Inqubo yokuthola: Amahora angu-3 (Ngaphandle kwenqubo eyenziwa ngesandla)
I-TAGMe DNA Methylation Detection Kits (qPCR) ye-Endometrial Cancer
| Isicelo somtholampilo | Ukuxilongwa komtholampilo kwe-endometrial carcinoma |
| Ufuzo lokutholwa | PCDHGB7 |
| Uhlobo lwesampula | Izibonelo zomlomo wesibeletho zabesifazane |
| Indlela yokuhlola | I-Fluorescence quantitative PCR technology |
| Amamodeli asebenzayo | ABI7500 |
| Ukucaciswa kokupakisha | 48 izivivinyo/ikhithi |
| Izimo Zokugcina | Ikhithi A kufanele igcinwe ku-2-30 ℃ Ikhithi B kufanele igcinwe ku -20±5℃ Ivumeleke kufika ezinyangeni eziyi-12. |
Mayelana NATHI
I-Epiprobe inokwakhiwa kwengqalasizinda ebanzi: Isikhungo sokukhiqiza se-GMP sihlanganisa indawo engamamitha-skwele angama-2200, futhi sigcina uhlelo lokuphatha ikhwalithi ye-ISO13485, oluhlangabezana nezidingo zokukhiqiza zazo zonke izinhlobo zemikhiqizo yokuhlola izakhi zofuzo;ilabhorethri yezokwelapha ihlanganisa indawo engamamitha-skwele angama-5400 futhi inamandla okwenza ibhizinisi lokutholwa komdlavuza njengelabhorethri yezokwelapha ephuma eceleni eqinisekisiwe.Ngaphandle kwalokho, sinemikhiqizo emithathu ethole isitifiketi se-CE, emboza umdlavuza womlomo wesibeletho, umdlavuza we-endometrial kanye nokutholwa okuhlobene nomdlavuza we-urothelial.
Ubuchwepheshe bokutholwa komdlavuza we-Epiprobe bungasetshenziswa ekuhlolweni komdlavuza kusenesikhathi, ukuxilongwa okusizayo, ukuhlolwa kwangaphambi kokuhlinzwa kanye nokuhlolwa kwangemva kokuhlinzwa, ukuqapha kabusha kwe-recrudescence, okuqhutshwa kuyo yonke inqubo yokuxilongwa nokwelashwa komdlavuza, ukuhlinzeka ngezixazululo ezingcono kodokotela neziguli.
