I-TAGMe DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
IZICI ZOMKHIQIZO
Ukunemba
Kugunyazwe amasampula omtholampilo angaphezu kuka-3500 ocwaningweni lwezikhungo eziningi ezingaboni kabili, umkhiqizo unokucaciswa okungu-92.7% kanye nokuzwela okungama-82.1%.
Elula
Ubuchwepheshe bokuqala bokutholwa kwe-Me-qPCR bungaqedwa ngesinyathelo esisodwa phakathi namahora angu-3 ngaphandle kokuguqulwa kwe-bisulfite.
Ayihlaseleki
Kudingeka u-30 ml kuphela wesampula yomchamo ukuze kutholwe izinhlobo ezi-3 zomdlavuza, okuhlanganisa umdlavuza wezinso, umdlavuza wesinye, umdlavuza wesinye ngesikhathi esifanayo.
Izimo zohlelo lokusebenza
Ukuxilongwa kokusiza: Iziguli ezinomdlavuza we-urothelial zingahlolwa ngendlela engahlanyisi ukusiza ekuxilongweni komtholampilo.
Ukuhlolwa kokuphumelela kokuhlinzwa/chemotherapy: Linganisa ukusebenza kahle kokuhlinzwa/ ngamakhemikhali ukuze usize ukuthuthukiswa komtholampilo komphumela wokwelapha.
Ukuqapha ukuphinda kwesibalo sabantu ngemuva kokuhlinzwa:Iziguli ezinomdlavuza we-urothelial zingaqashelwa ukuze ziphinde ziphinde ngendlela engahlaseli ngendlela engahlaseli, ethuthukisa ukuthobela kwesiguli.
Iqoqo lesampula
Indlela yesampula: Indlela yokuthatha amasampula: Qoqa isampula lomchamo (umchamo wasekuseni noma umchamo ongahleliwe), engeza isixazululo esigcina umchamo bese uxuba kahle, usigcine endaweni efudumele bese ulebula ukuze uhlolwe okulandelayo.
Ukulondolozwa kwamasampuli: Amasampuli angagcinwa ekamelweni lokushisa kuze kube yizinsuku eziyi-14, ku-2-8 ℃ kuze kufike ezinyangeni ezi-2, futhi ku-20±5℃ kuze kufike ezinyangeni ezingu-24.
Inqubo yokuthola: Amahora angu-3 (Ngaphandle kwenqubo eyenziwa ngesandla)
I-DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
| Isicelo somtholampilo | Ukuxilongwa komtholampilo kwe-cnacer urothelial;ukuhlolwa kokuphumelela kokwelashwa kokuhlinzwa/ikhemotherayi;ukuqapha ukuphindaphinda kwangemva kokuhlinzwa |
| Ufuzo lokutholwa | UC |
| Uhlobo lwesampula | Isampula yeseli ye-urine exfoliated (i-urine sediment) |
| Indlela yokuhlola | I-Fluorescence quantitative PCR technology |
| Amamodeli asebenzayo | ABI7500 |
| Ukucaciswa kokupakisha | 48 izivivinyo/ikhithi |
| Izimo Zokugcina | Ikhithi A kufanele igcinwe ku-2-30 ℃Ikhithi B kufanele igcinwe ku -20±5℃ Ivumeleke kufika ezinyangeni eziyi-12. |
