I-TAGMe DNA Methylation Detection Kits(qPCR) yomdlavuza womlomo wesibeletho
IZICI ZOMKHIQIZO
Ukunemba
Kugunyazwe amasampula omtholampilo angaphezu kuka-36000 ocwaningweni lwezikhungo eziningi eziyizimpumputhe ezimbili, umkhiqizo unokucaciswa okungu-94.3% kanye nokuzwela okungama-96.0%.
Elula
Ubuchwepheshe bokuqala bokutholwa kwe-Me-qPCR bungaqedwa ngesinyathelo esisodwa phakathi namahora angu-3 ngaphandle kokuguqulwa kwe-bisulfite.
Ngaphambili
Ukuhlolwa komdlavuza womlomo wesibeletho kungathuthukiswa kuze kufike esigabeni sezilonda ezisezingeni eliphezulu (izilonda ezidala umdlavuza).
Okuzenzakalelayo
Ihambisana nesofthiwe yokuhlaziya imiphumela eyenziwe ngokwezifiso, ukuchazwa kwemiphumela kuyazenzakalela futhi kuyafundeka ngokuqondile.
UKUSETSHENZISWA OKUHLOSIWE
Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative ye-hypermethylation yofuzo lwe-PCDHGB7 kumasampula omlomo wesibeletho.Umphumela omuhle ubonisa ubungozi obukhulayo bebanga lesi-2 noma lebanga eliphezulu/elithuthuke kakhulu le-intraepithelial neoplasia yomlomo wesibeletho (CIN2+, okuhlanganisa i-CIN2, CIN3, i-adenocarcinoma in situ, kanye nomdlavuza womlomo wesibeletho), okudinga ukuhlolwa okwengeziwe kwe-colposcopy kanye/noma i-histopathological.Ngokuphambene, imiphumela yokuhlolwa engemihle ibonisa ukuthi ubungozi be-CIN2+ buphansi, kodwa ubungozi abukwazi ukukhishwa ngaphandle ngokuphelele.Ukuxilongwa kokugcina kufanele kusekelwe ku-colposcopy kanye/noma imiphumela ye-histopathological.I-PCDHGB7 iyilungu lomndeni we-protocadherin γ gene cluster.I-Protocadherin itholwe ukuthi ilawula izinqubo zebhayoloji ezifana nokwanda kwamangqamuzana, umjikelezo wamaseli, i-apoptosis, ukuhlasela, ukufuduka kanye ne-autophagy yamangqamuzana e-tumor ngokusebenzisa izindlela ezihlukahlukene zokubonisa, futhi ukuthuliswa kofuzo okubangelwa i-hypermethylation yesifunda somgqugquzeli kuhlobene eduze nokwenzeka nokuthuthuka. eminingi yomdlavuza.Kubikwa ukuthi i-hypermethylation ye-PCDHGB7 ihlotshaniswa nezinhlobonhlobo zezimila, njenge-non-Hodgkin lymphoma, umdlavuza webele, umdlavuza womlomo wesibeletho, umdlavuza we-endometrial kanye nomdlavuza wesinye.
ISIMISO SOKUTHOLA
Le khithi iqukethe i-nucleic acid extract reagent kanye ne-PCR yokuthola i-reagent.I-Nucleic acid ikhishwa ngendlela esekelwe ku-magnetic-bead.Le khithi isekelwe kumgomo wendlela ye-PCR yobuningi be-fluorescence, kusetshenziswa ukusabela kwe-PCR ye-methylation-specific real-time ukuhlaziya i-DNA yesifanekiso, kanye nokuthola amasayithi e-CpG yofuzo lwe-PCDHGB7 kanye nomaka wokulawula ikhwalithi wangaphakathi wezingcezu zofuzo lwereferensi i-G1 ne-G2.Izinga le-methylation le-PCDHGB7 kusampula, noma inani le-Me, libalwa ngokuvumelana nevelu ye-Ct ye-PCDHGB7 yofuzo lwe-methylated DNA amplification kanye nenani le-Ct lereferensi.I-PCDHGB7 gene hypermethylation isimo esihle noma esingalungile sinqunywa ngokwevelu Yami.
Izimo zohlelo lokusebenza
Ukuhlolwa kusenesikhathi
Abantu abanempilo
Ukuhlolwa Kwengozi Yomdlavuza
Isibalo sabantu abasengozini enkulu (esilungele i-human papillomavirus (hrHPV) engcuphe kakhulu noma i-positive for cytology yokukhishwa komlomo wesibeletho)
Ukuqapha Ukuphindaphinda
Isibalo sabantu abangemva kokuhlinzwa (abanomlando wezilonda zomlomo wesibeletho ezisezingeni eliphezulu noma umdlavuza womlomo wesibeletho)
Ukubaluleka komtholampilo
Ukuhlolwa kusenesikhathi kwabantu abanempilo:Umdlavuza womlomo wesibeletho kanye nezilonda eziyingozi zingahlolwa ngokunembile
Ukuhlolwa kobungozi kubantu abasengozini enkulu:Ukuhlukaniswa kwengozi kungenziwa kubantu abane-HPV ukuze kuqondiswe ukutholwa kwe-triage elandelayo
Ukuqapha ukuphindeka kwabantu bangemva kokuhlinzwa:Ukuqapha kokuphindaphinda kwenani labantu kwangemva kokuhlinzwa kungenziwa ukuze kuvinjelwe ukubambezeleka kokwelashwa okubangelwa ukuphindaphinda
Iqoqo lesampula
Indlela yesampula: Beka isampula yomlomo wesibeletho esilahlwayo endaweni yomlomo wesibeletho, hlikihla kahle ibhulashi lomlomo wesibeletho bese uphendukisa izikhathi ezi-4-5 ngokwewashi, khipha kancane ibhulashi lomlomo wesibeletho, ulifake kusisombululo sokulondoloza amaseli, bese ulilebula ukuze kuhlolwe okulandelayo.
Ukulondolozwa kwamasampuli:Amasampuli angagcinwa ekamelweni lokushisa kuze kube yizinsuku eziyi-14, ku-2-8 ℃ kuze kufike ezinyangeni ezi-2, futhi ku-20±5℃ kuze kufike ezinyangeni ezingu-24.
Inqubo yokuthola: Amahora angu-3 (Ngaphandle kwenqubo eyenziwa ngesandla)
I-DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
| Isicelo somtholampilo | Ukuxilongwa komtholampilo komdlavuza womlomo wesibeletho |
| Ufuzo lokutholwa | PCDHGB7 |
| Uhlobo lwesampula | Izibonelo zomlomo wesibeletho zabesifazane |
| Indlela yokuhlola | I-Fluorescence quantitative PCR technology |
| Imodeli esebenzayo | ABI7500 |
| Ukucaciswa kokupakisha | 48 izivivinyo/ikhithi |
| Izimo Zokugcina | Ikhithi A kufanele igcinwe ku-2-30 ℃ Ikhithi B kufanele igcinwe ku -20±5℃ Ivumeleke kufika ezinyangeni eziyi-12 |
