Ikhithi yokukhipha i-Nucleic Acid (A01)
Umgomo wokutholwa
Ngemva kokukhipha i-DNA ye-genomic ngokuhlukanisa amaseli nge-lysis buffer, ubuhlalu obuzibuthe bungabopha ngokukhetha i-genomic DNA kusampula.Inani elincane lokungcola elimuncwa ubuhlalu obuzibuthe lingasuswa isigcinalwazi sokugeza.Ku-TE, ubuhlalu obuzibuthe bungakhulula i-DNA ye-boundgenome, buthole i-DNA yezinga eliphezulu yofuzo.Le ndlela ilula futhi iyashesha futhi izinga le-DNA ekhishiwe liphezulu, elingahlangabezana nemfuneko yokutholwa kwe-DNA methylation.Ngaleso sikhathi, ikhithi yokukhipha esekelwe kubuhlalu obuzibuthe ingase ihambisane nokukhipha i-nucleic acid okuzenzakalelayo, ukuhlangabezana nemisebenzi yokukhipha i-nucleic acid ephezulu.
Izingxenye eziyinhloko ze-reagent
Izingxenye ziboniswa kuthebula 1:
Ithebula 1 Izingxenye Ze-reagent futhi Iyalayisha
| Igama lengxenye | Izingxenye eziyinhloko | Usayizi (48) | Usayizi (200) |
| 1. Isixazululo se-Lysis | I-Guanidine hydrochloride, i-Tris | 11 mL/ibhodlela | 44 mL/ibhodlela |
| 2. Izixazululo zokuhlanza A | NaCl, Tris | 11 mL/ibhodlela | 44 mL/ibhodlela |
| 3. Izixazululo zokuhlanza B | NaCl, Tris | 13 mL/ibhodlela | 26.5mL/ibhodlela *2 |
| 4. Eluent | Tris, EDTA | 12 mL/ibhodlela | 44 mL/ibhodlela |
| 5. Isixazululo se-Protease K | UProtease K | 1.1mL/ucezu | 4.4mL/ucezu |
| 6. Ukumiswa kobuhlalu obuzibuthe 1 | Ubuhlalu kazibuthe | 1.1mL/ucezu | 4.4mL/ucezu |
| 7. Imiyalo yokukhipha ama-nucleic acid reagent |
| 1 ikhophi | 1 ikhophi |
Izingxenye ezidingekayo ekukhishweni kwe-nucleic acid, kodwa azifakiwe kukhithi:
1. I-Reagent: I-ethanol e-anhydrous, isopropanol ne-PBS;
2. Izinto ezisetshenziswayo: 50ml centrifuge tube kanye ne-1.5ml EP tube;
3. Izisetshenziswa: Iketela lokugeza amanzi, i-pipettor, ishalofu kazibuthe, i-centrifuge, ipuleti elijulile elinezimbobo ezingama-96 (okuzenzakalelayo), okokusebenza okuzenzakalelayo kokukhipha i-nucleic acid (okuzenzakalelayo).
Ulwazi oluyisisekelo
Izidingo zesampula
1. Ukungcoliswa kwesiphambano phakathi kwamasampuli kufanele kugwenywe ekuqoqweni nasekugcinweni kwesampula.
2.Ukutholwa kuzoqedwa ngaphansi kwesitoreji sezinsuku ezingu-7 sezinga lokushisa le-ambient ngemva kokuqoqwa kwesampula yeseli ye-cervical exfoliated (okungagxilile).Ukutholwa kuzoqedwa ngaphansi kokugcinwa kwezinsuku ezingama-30 kwezinga lokushisa elizungezile ngemva kokuqoqwa kwesifanekiso somchamo;Ukutholwa kuzoqedwa ngesikhathi ngemuva kokuqoqwa kwamasampula eseli akhulisiwe.
Ukucaciswa kokupaka:200pcs/ibhokisi, 48pcs/ibhokisi.
Izimo zesitoreji:2-30 ℃
Isikhathi sokuqinisekisa:Izinyanga ezingu-12
Idivayisi esebenzayo:I-Tianlong NP968-C ithuluzi lokukhipha i-nucleic acid, ithuluzi lokukhipha i-nucleic acid ye-Tiangen TGuide S96, i-GENE DIAN EB-1000 ithuluzi lokukhipha i-nucleic acid.
Isitifiketi serekhodi ledivayisi yezokwelapha Inombolo/imfuneko yobuchwepheshe yomkhiqizo No.: HJXB No. 20210099.
Usuku lokugunyazwa nokuguqulwa kwemiyalo:
Idethi yokugunyaza: Nov. 18, 2021
