I-TAGMe DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
IZICI ZOMKHIQIZO
Ukunemba
Kugunyazwe amasampula omtholampilo angaphezu kuka-3500 ocwaningweni lwezikhungo eziningi ezingaboni kabili, umkhiqizo unokucaciswa okungu-92.7% kanye nokuzwela okungama-82.1%.
Elula
Ubuchwepheshe bokuqala bokutholwa kwe-Me-qPCR bungaqedwa ngesinyathelo esisodwa phakathi namahora angu-3 ngaphandle kokuguqulwa kwe-bisulfite.
Ayihlaseleki
Kudingeka u-30 ml kuphela wesampula yomchamo ukuze kutholwe izinhlobo ezi-3 zomdlavuza, okuhlanganisa umdlavuza wezinso, umdlavuza wesinye, umdlavuza wesinye ngesikhathi esifanayo.
Izimo zohlelo lokusebenza
Ukuxilongwa Okusizayo
Abantu abahlushwa i-hematuria engenabuhlungu/ okusolwa ukuthi bane-urothelial (umdlavuza wesibeletho/ umdlavuza we-renal pelvis)
Ukuhlolwa Kwengozi Yomdlavuza
Ukuhlinzwa/ukwelashwa ngamakhemikhali-okudinga abantu abane-urothelial carcinoma;
Ukuqapha Ukuphindaphinda
Isibalo se-postoperative ene-urothelial carcinoma
UKUSETSHENZISWA OKUHLOSIWE
Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative ye-hypermethylation ye-Urothelial Carcinoma (UC) isakhi sofuzo ku-urothelial specimens.Umphumela omuhle ukhombisa ukwanda kwengozi ye-UC, edinga i-cystoscope eyengeziwe kanye/noma ukuhlolwa kwe-histopathological.Ngokuphambene nalokho, imiphumela yokuhlolwa engemihle ibonisa ukuthi ubungozi be-UC buphansi, kodwa ubungozi abukwazi ukukhishwa ngaphandle ngokuphelele.Ukuxilongwa kokugcina kufanele kusekelwe ku-cystoscope kanye/noma imiphumela ye-histopathological.
ISIMISO SOKUTHOLA
Le khithi iqukethe i-nucleic acid extract reagent kanye ne-PCR yokuthola i-reagent.I-Nucleic acid ikhishwa ngendlela esekelwe ku-magnetic-bead.Le khithi isekelwe kumgomo we-fluorescence quantitative PCR indlela, kusetshenziswa i-methylation-specific real-time PCR reaction ukuze kuhlaziywe i-DNA yesifanekiso, kanye nokuthola amasayithi e-CpG ofuzo lwe-UC kanye nomaka wokulawula ikhwalithi wangaphakathi wezingcezu zofuzo lwereferensi i-G1 ne-G2.Izinga le-methylation lesakhi sofuzo se-UC, elibizwa ngokuthi inani Lami, libalwa ngokuvumelana nenani le-Ct le-UC le-methylated DNA amplification kanye nenani le-Ct lereferensi.I-UC gene hypermethylation isimo esihle noma esingalungile sinqunywa ngokwevelu ye-Me.
I-DNA Methylation Detection Kits (qPCR) ye-Urothelial Cancer
| Isicelo somtholampilo | Ukuxilongwa komtholampilo kwe-cnacer urothelial;ukuhlolwa kokuphumelela kokwelashwa kokuhlinzwa/ikhemotherayi;ukuqapha ukuphindaphinda kwangemva kokuhlinzwa |
| Ufuzo lokutholwa | UC |
| Uhlobo lwesampula | Isampula yeseli ye-urine exfoliated (i-urine sediment) |
| Indlela yokuhlola | I-Fluorescence quantitative PCR technology |
| Amamodeli asebenzayo | ABI7500 |
| Ukucaciswa kokupakisha | 48 izivivinyo/ikhithi |
| Izimo Zokugcina | Ikhithi A kufanele igcinwe ku-2-30 ℃ Ikhithi B kufanele igcinwe ku -20±5℃ Ivumeleke kufika ezinyangeni eziyi-12. |
