I-TAGMe DNA Methylation Detection Kits (qPCR) yomdlavuza wesibeletho / umdlavuza we-Endometrial
IZICI ZOMKHIQIZO
Ayihlaseleki
Isebenza ngebhulashi lomlomo wesibeletho kanye namasampula e-Pap smear.
Elula
Ubuchwepheshe bokuqala bokutholwa kwe-Me-qPCR bungaqedwa ngesinyathelo esisodwa phakathi namahora angu-3 ngaphandle kokuguqulwa kwe-bisulfite.
Ngaphambili
Iyabonakala esigabeni esiyingozi.
Okuzenzakalelayo
Ihambisana nesofthiwe yokuhlaziya imiphumela eyenziwe ngokwezifiso, ukuchazwa kwemiphumela kuyazenzakalela futhi kuyafundeka ngokuqondile.
Izimo zohlelo lokusebenza
Ukuhlolwa kusenesikhathi
Abantu abanempilo
Ukuhlolwa Kwengozi Yomdlavuza
Isibalo sabantu abasengcupheni ephezulu (esingengozini enkulu ye-human papillomavirus (hrHPV) noma i-cytology ye-cervical exfoliation / i-positive ku-human papillomavirus (hrHPV) enobungozi obukhulu noma i-cytology yokukhishwa komlomo wesibeletho)
Ukuqapha Ukuphindaphinda
Inani labantu ababikezelayo
UKUSETSHENZISWA OKUHLOSIWE
Le kit isetshenziselwa ukutholwa kwe-in vitro qualitative ye-hypermethylation yofuzo lwe-PCDHGB7 kumasampula omlomo wesibeletho.Ngomdlavuza womlomo wesibeletho, umphumela omuhle ubonisa ubungozi obukhulayo bebanga lesi-2 noma lebanga eliphezulu/elithuthuke kakhulu le-intraepithelial neoplasia yomlomo wesibeletho (CIN2+, kuhlanganise i-CIN2, i-CIN3, i-adenocarcinoma in situ, kanye nomdlavuza womlomo wesibeletho), okudinga i-colposcopy eyengeziwe kanye/noma ukuhlolwa kwe-histopathological. .Ngokuphambene, imiphumela yokuhlolwa engemihle ibonisa ukuthi ubungozi be-CIN2+ buphansi, kodwa ubungozi abukwazi ukukhishwa ngaphandle ngokuphelele.Ukuxilongwa kokugcina kufanele kusekelwe ku-colposcopy kanye/noma imiphumela ye-histopathological.Ngaphezu kwalokho, ngomdlavuza we-endometrial, umphumela omuhle ubonisa ingozi eyengeziwe yezilonda ze-endometrial precancerous kanye nomdlavuza, okudinga ukuhlolwa okuqhubekayo kwe-histopathological ye-endometrium.Ngokuphambene nalokho, imiphumela yokuhlolwa engemihle ibonisa ukuthi ingozi yezilonda ze-endometrial precancerous kanye nomdlavuza iphansi, kodwa ingozi ayikwazi ukukhishwa ngokuphelele.Ukuxilongwa kokugcina kufanele kusekelwe emiphumeleni yokuhlolwa kwe-histopathological ye-endometrium.
I-PCDHGB7 iyilungu lomndeni we-protocadherin γ gene cluster.I-Protocadherin itholwe ukuthi ilawula izinqubo zebhayoloji ezifana nokwanda kwamangqamuzana, umjikelezo wamaseli, i-apoptosis, ukuhlasela, ukufuduka kanye ne-autophagy yamangqamuzana e-tumor ngokusebenzisa izindlela ezihlukahlukene zokubonisa, futhi ukuthuliswa kofuzo okubangelwa i-hypermethylation yesifunda somgqugquzeli kuhlobene eduze nokwenzeka nokuthuthuka. eminingi yomdlavuza.Kubikwa ukuthi i-hypermethylation ye-PCDHGB7 ihlotshaniswa nezinhlobonhlobo zezimila, njenge-non-Hodgkin lymphoma, umdlavuza webele, umdlavuza womlomo wesibeletho, umdlavuza we-endometrial kanye nomdlavuza wesinye.
ISIMISO SOKUTHOLA
Le khithi iqukethe i-nucleic acid extract reagent kanye ne-PCR yokuthola i-reagent.I-Nucleic acid ikhishwa ngendlela esekelwe ku-magnetic-bead.Le khithi isekelwe kumgomo wendlela ye-PCR yobuningi be-fluorescence, kusetshenziswa ukusabela kwe-PCR ye-methylation-specific real-time ukuhlaziya i-DNA yesifanekiso, kanye nokuthola amasayithi e-CpG yofuzo lwe-PCDHGB7 kanye nomaka wokulawula ikhwalithi wangaphakathi wezingcezu zofuzo lwereferensi i-G1 ne-G2.Izinga le-methylation le-PCDHGB7 kusampula, noma inani le-Me, libalwa ngokuvumelana nevelu ye-Ct ye-PCDHGB7 yofuzo lwe-methylated DNA amplification kanye nenani le-Ct lereferensi.I-PCDHGB7 gene hypermethylation isimo esihle noma esingalungile sinqunywa ngokwevelu Yami.
