TAGMe DNA Methylation Detection for Pan-cancer
Izici zomkhiqizo
Ukwelashwa okuhlaselayo kancane:Khipha okungenani u-3mL wegazi le-venous noma ezinye izinhlobo zesampula zomtholampilo ezifanele
Ukunemba:Yamukela idatha enkulu kanye ne-algorithm ye-AI, ukucaciswa kokuhlolwa≥95%
Ukuhlukahluka:Ukutholwa okukodwa kungamboza izimila eziyingozi ezingama-25.
Isigaba sokuqala:Ngenelela ekuhlolweni komdlavuza kusenesikhathi, kuyo yonke inqubo yokuxilongwa nokwelashwa kwesimila.
Ukufaneleka:Ukwamukela izindinganiso zegolide zokutholwa kwe-methylation - ubuchwepheshe bokulandelana kwe-pyrophosphate, kungaqedela ukuhlolwa phakathi namahora angu-4.
Igunya:Ngamalungelo obunikazi angama-54 amazwe ngamazwe nawasekhaya, imiphumela yocwaningo efanele ishicilelwe kuCancer Research, Genome Research kanye nabanye omagazini abadumile bamazwe ngamazwe.
Isimiso Sokuthola
Ukutholwa komdlavuza wonke imikhiqizo yokuhlola ye-plasma ctDNA methylation eyakhiwe yi-TAGMe, edinga okungenani u-3ml wegazi eliphelele ukuze ithwebule ngempumelelo futhi inqume isimo se-methylation sezindawo ezikhethekile zokumisa ze-ctDNA, ukuze kufezwe ukuhlolwa kusenesikhathi nokuqapha okunembile. yesimila.
Ukusebenza
Ekuqinisekisweni kwamasampula omtholampilo angaphezu kuka-3000, izinga le-methylation le-TAGMe ezicutshini zesimila laliphezulu kakhulu kunalelo lezicubu ezivamile.Ngakho-ke, i-TAGMe ingasetshenziswa njengomaka womdlavuza we-pan-cancer ukuze kutholwe izinhlobo zomdlavuza.
Izakhi zofuzo ezihlobene ne-histone zine-hypermethylated kumdlavuza wamaphaphu futhi i-hypermethylated HIST1H4F ingasebenza njenge-pan-cancer biomarker.
--- Ucwaningo Lomdlavuza, IF: 12.7
Njengomaka womdlavuza we-pan-cancer, i-TAGMe inokucaciswa okuphezulu nokuzwela ekuqinisekiseni isampula yomtholampilo, enereferensi yenani elibalulekile ekuxilongweni komtholampilo kwamathumba.
I-TAGMe iyazwela kumasampuli ahlukene wesimila uma ukucaciswa kungaphezu kuka-95%,
Izimo zohlelo lokusebenza
Umlando womndeni womdlavuza:Iziguli ezinomdlavuza ezihlotsheni eziseduze noma ezibambisanayo.
Ukutheleleka ngegciwane kanye nezifo ezingamahlalakhona:Njenge-HPV, i-HBV nezinye izifo ezibangelwa amagciwane, ama-polyps amathumbu, i-atrophic gastritis engapheli kanye ne-cirrhosis yesibindi, njll.
Kuvezwe endaweni ye-carcinogenic isikhathi eside:Kuchayeke emvelweni wemisebe noma kumakhemikhali ayingozi isikhathi eside.
Uphawu lwesimila esisolisayo:njengokopha okungajwayelekile kwesitho sangasese sowesifazane, i-hematochezia, umchamo, izigaxa zamaphaphu, umkhuhlane ophansi noma ukuwohloka okungachazwanga, njll.
Imikhuba emibi yokuphila yesikhathi eside: Ukubhema isikhathi eside noma ukubhema ugwayi, ukuphuza ngokweqile, ukudla okushisayo noma ukuphuza okushisayo, ukudla usawoti omningi, okufakwe emanzini anosawoti, okukhuntile, ukudla okubhenyiwe nokuthosiwe, njll.
Naka izimo zakho ezinempilo:ikakhulukazi eqenjini elineminyaka engaphezu kuka-40
