I-Gargle Nucleic Acid Extraction Reagents
ISIMISO SOKUTHOLA
Umkhiqizo ikakhulukazi uqukethe i-gargle, ingxube ye-superparamagnetic nanospheres emunca kakhulu, kanye nama-lysis reagents ahlukile.Ubuhlalu obukazibuthe obushumekiwe obuhlukile bunokuhambisana okuhle kwezingxenye zomzimba (okuhlanganisa amagciwane amahhala namaseli atheleleke ngegciwane).Lapho uthintana nesisombululo se-lysis, i-non-ionic cell/nucleus-membrane-breaking surfactants kanye nama-protease inhibitors esixazululweni angavimbela umsebenzi we-DNA/RNA enzyme, futhi azinzise i-nucleic acid.Zonke izinto ze-nucleic acid ezingxenyeni zomzimba ze-gargle zikhishwa ngokuphumelelayo kwisisombululo se-lysis, ngokushesha zithole i-nucleic acid.Amasampula e-gargle asebenzisa le kit awadingi ukuhlanzwa kwe-nucleic acid, engasebenza ngokuqondile kukhithi yokutholwa kwe-nucleic acid engezansi komfula.
Izingxenye eziyinhloko ze-reagent
Izingxenye ziboniswa kuthebula 1.
Ithebula 1 Izingxenye Futhi Iyalayisha Kukhithi
| Igama lengxenye | Izingxenye eziyinhloko | Usayizi (1) | Usayizi (10) | Usayizi (30) | Usayizi (50) |
| 1. Gargle A | NaCl | 8mL/ithubhu | 8mL/ishubhu *10 amashubhu | 8mL/ishubhu *30 amashubhu | 8mL/ishubhu *50 amashubhu |
| 2. Umqoqi we-Gargle | PP | 1 isiqephu | 10 ama-pcs | 30 ama-pcs | 50 ama-pcs |
| 3. Isixazululo Sokucebisa B | Ubuhlalu kazibuthe | 2mL/ithubhu | 2mL/ishubhu *10 amashubhu | 2mL/ishubhu *30 amashubhu | 2mL/ishubhu *50 amashubhu |
| 4 ULysis Buffer C | UProtease K | 0.2mL/ isiqephu | 0.2mL/ucezu*10 ama-pcs | 0.2mL/ucezu*30 ama-pcs | 0.2mL/ucezu*50 ama-pcs |
| 5. I-Magnetic Cap | Umazibuthe | 1 isiqephu | 10 ama-pcs | 30 ama-pcs | 50 ama-pcs |
Izingxenye zisebenza izinyanga eziyi-12.
Izingxenye ezidingekayo ekukhishweni kwe-nucleic acid, kodwa azifakiwe kukhithi:
1. Izinto ezisetshenziswayo: 1.5ml EP tube;
2. Izinto zokusebenza: Ibhavu lamanzi (noma ukugeza kwensimbi), ama-pipettes, ne-centrifuge.
Ulwazi oluyisisekelo
Izimfuneko zesampula:
1. Kusebenza ekukhishweni kwe-nucleic acid kanye nokunothiswa kwamasampula e-gargle.
2. Isampula ye-gargle izokwengezwa kusixazululo sokucebisa B ngesikhathi ngemuva kokuqoqwa.Ubuhlalu obuzibuthe obubuyisiwe buzodluliselwa ku-lysis buffer C ngokushesha.Amasampuli angezwe ku-lysis buffer C angagcinwa ekamelweni lokushisa.
Ukucaciswa kokupakisha: 1 ucezu/ibhokisi, 10pcs/ibhokisi, 30pcs/ibhokisi, futhi 50pcs/ibhokisi.
Izimo zesitoreji: Isixazululo sokucebisa B kanye nekhambi le-lysis C kufanele kugcinwe izinyanga eziyi-12 ku-2-8℃, kanti ezinye izingxenye zingagcinwa izinyanga eziyi-12 ngaphansi kwezinga lokushisa lekamelo;Ikhithi ingathuthwa futhi igcinwe isikhashana ngaphansi kwezinga lokushisa le-ambient, elingeke lidlule izinsuku ezi-5.
Isikhathi sokuqinisekisa: Izinyanga ezingu-12
Isitifiketi serekhodi ledivayisi yezokwelapha Inombolo/imfuneko yobuchwepheshe yomkhiqizo No.:HJXB No. 20220086.
Usuku lokugunyazwa nokubuyekezwa Kwemiyalo:
Idethi yokugunyaza: Oct. 26, 2022
